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Mendeley readers
Chapter title |
Revealing Sister Chromatid Interactions with the loxP/ Cre Recombination Assay
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Chapter number | 3 |
Book title |
The Bacterial Nucleoid
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7098-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7097-1, 978-1-4939-7098-8
|
Authors |
Elise Vickridge, Charlène Planchenault, Olivier Espéli, Vickridge, Elise, Planchenault, Charlène, Espéli, Olivier |
Abstract |
Sister chromatid cohesion is a transient state during replication in bacteria. It has been recently demonstrated that the extent of contact between cohesive sisters during the cell cycle is dependent on topoisomerase IV activity, suggesting that topological links hold sister chromatids together. In the present protocol, we describe a simple method to quantify the frequency of the contacts between two cohesive sister chromatids. This method relies on a site specific recombination assay between loxP sites upon Cre induction. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 25% |
Professor > Associate Professor | 1 | 25% |
Researcher | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 75% |
Unknown | 1 | 25% |