| Chapter title |
Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins
|
|---|---|
| Chapter number | 11 |
| Book title |
The Bacterial Nucleoid
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7098-8_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7097-1, 978-1-4939-7098-8
|
| Authors |
Ramon A. van der Valk, Niels Laurens, Remus T. Dame, Valk, Ramon A. van der, Laurens, Niels, Dame, Remus T. |
| Abstract |
Architectural DNA binding proteins are key to the organization and compaction of genomic DNA inside cells. Tethered Particle Motion (TPM) permits analysis of DNA conformation and detection of changes in conformation induced by such proteins at the single molecule level in vitro. As many individual protein-DNA complexes can be investigated in parallel, these experiments have high throughput. TPM is therefore well suited for characterization of the effects of protein-DNA stoichiometry and changes in physicochemical conditions (pH, osmolarity, and temperature). Here, we describe in detail how to perform Tethered Particle Motion experiments on complexes between DNA and architectural proteins to determine their structural and biochemical characteristics. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 29% |
| Student > Master | 3 | 21% |
| Lecturer | 1 | 7% |
| Student > Ph. D. Student | 1 | 7% |
| Professor > Associate Professor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 4 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 43% |
| Agricultural and Biological Sciences | 3 | 21% |
| Physics and Astronomy | 1 | 7% |
| Unknown | 4 | 29% |