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The Bacterial Nucleoid

Overview of attention for book
Cover of 'The Bacterial Nucleoid'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli
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    Chapter 2 Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes
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    Chapter 3 Revealing Sister Chromatid Interactions with the loxP/ Cre Recombination Assay
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    Chapter 4 Transposon Insertion Site Sequencing for Synthetic Lethal Screening
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    Chapter 5 WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites
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    Chapter 6 High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing
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    Chapter 7 Generation and Analysis of Chromosomal Contact Maps of Bacteria
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    Chapter 8 Nucleoid-Associated Proteins: Genome Level Occupancy and Expression Analysis
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    Chapter 9 Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins
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    Chapter 10 A Chromosome Co-Entrapment Assay to Study Topological Protein–DNA Interactions
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    Chapter 11 Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins
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    Chapter 12 Biochemical Analysis of Bacterial Condensins
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    Chapter 13 Exploring Condensins with Magnetic Tweezers
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    Chapter 14 Applications of Magnetic Tweezers to Studies of NAPs
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    Chapter 15 A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data
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    Chapter 16 Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization
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    Chapter 17 Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage
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    Chapter 18 Measuring In Vivo Protein Dynamics Throughout the Cell Cycle Using Microfluidics
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    Chapter 19 Imaging of Bacterial Chromosome Organization by 3D Super-Resolution Microscopy
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    Chapter 20 Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins: The Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
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    Chapter 21 Procedures for Model-Guided Data Analysis of Chromosomal Loci Dynamics at Short Time Scales
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    Chapter 22 Isolation and Characterization of Bacterial Nucleoids in Microfluidic Devices
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    Chapter 23 Modeling Bacterial DNA: Simulation of Self-Avoiding Supercoiled Worm-Like Chains Including Structural Transitions of the Helix
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    Chapter 24 Molecular Dynamics Simulation of Supercoiled, Knotted, and Catenated DNA Molecules, Including Modeling of Action of DNA Gyrase
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    Chapter 25 Erratum to: Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins, the Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
Attention for Chapter 1: Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli
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Chapter title
Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli
Chapter number 1
Book title
The Bacterial Nucleoid
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7098-8_1
Pubmed ID
Book ISBNs
978-1-4939-7097-1, 978-1-4939-7098-8
Authors

Jack A. Bryant, David J. Lee, Bryant, Jack A., Lee, David J.

Abstract

Homologous recombination methods enable modifications to be made to the bacterial chromosome. Commonly, the λ phage RED proteins are employed as a site-specific recombinase system, to facilitate recombination of linear DNA fragments with targeted regions of the chromosome. Here we describe methods for the efficient delivery of linear DNA segments containing homology to the chromosome into the cell as substrates for the λRED proteins. Combined with antibiotic selection and counterselection, we demonstrate that using this method facilitates accurate, rapid editing of the chromosome.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2018.
All research outputs
#14,980,451
of 23,043,346 outputs
Outputs from Methods in molecular biology
#4,741
of 13,194 outputs
Outputs of similar age
#243,616
of 421,409 outputs
Outputs of similar age from Methods in molecular biology
#416
of 1,074 outputs
Altmetric has tracked 23,043,346 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,194 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,409 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.