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The Bacterial Nucleoid

Overview of attention for book
Cover of 'The Bacterial Nucleoid'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli
  3. Altmetric Badge
    Chapter 2 Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes
  4. Altmetric Badge
    Chapter 3 Revealing Sister Chromatid Interactions with the loxP/ Cre Recombination Assay
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    Chapter 4 Transposon Insertion Site Sequencing for Synthetic Lethal Screening
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    Chapter 5 WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites
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    Chapter 6 High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing
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    Chapter 7 Generation and Analysis of Chromosomal Contact Maps of Bacteria
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    Chapter 8 Nucleoid-Associated Proteins: Genome Level Occupancy and Expression Analysis
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    Chapter 9 Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins
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    Chapter 10 A Chromosome Co-Entrapment Assay to Study Topological Protein–DNA Interactions
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    Chapter 11 Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins
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    Chapter 12 Biochemical Analysis of Bacterial Condensins
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    Chapter 13 Exploring Condensins with Magnetic Tweezers
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    Chapter 14 Applications of Magnetic Tweezers to Studies of NAPs
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    Chapter 15 A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data
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    Chapter 16 Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization
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    Chapter 17 Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage
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    Chapter 18 Measuring In Vivo Protein Dynamics Throughout the Cell Cycle Using Microfluidics
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    Chapter 19 Imaging of Bacterial Chromosome Organization by 3D Super-Resolution Microscopy
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    Chapter 20 Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins: The Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
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    Chapter 21 Procedures for Model-Guided Data Analysis of Chromosomal Loci Dynamics at Short Time Scales
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    Chapter 22 Isolation and Characterization of Bacterial Nucleoids in Microfluidic Devices
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    Chapter 23 Modeling Bacterial DNA: Simulation of Self-Avoiding Supercoiled Worm-Like Chains Including Structural Transitions of the Helix
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    Chapter 24 Molecular Dynamics Simulation of Supercoiled, Knotted, and Catenated DNA Molecules, Including Modeling of Action of DNA Gyrase
  26. Altmetric Badge
    Chapter 25 Erratum to: Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins, the Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
Attention for Chapter 14: Applications of Magnetic Tweezers to Studies of NAPs
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Chapter title
Applications of Magnetic Tweezers to Studies of NAPs
Chapter number 14
Book title
The Bacterial Nucleoid
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7098-8_14
Pubmed ID
28842884
Book ISBNs
978-1-4939-7097-1, 978-1-4939-7098-8
Authors

Ricksen S. Winardhi, Jie Yan, Winardhi, Ricksen S., Yan, Jie

Abstract

Nucleoid-associated proteins (NAPs) are important factors in shaping bacterial nucleoid and regulating global gene expression. A great deal of insights into NAPs can be obtained through studies using single DNA molecule, which has been made possible owing to recent rapid development of single-DNA manipulation techniques. These studies provide information on modes of binding to DNA, which shed light on the mechanism underlying the regulatory function of NAPs. In addition, how NAPs organize DNA and thus their contribution to chromosomal DNA packaging can be determined. In this chapter, we introduce transverse magnetic tweezers that allows for convenient manipulation of long DNA molecules, and its applications in studies of NAPs as exemplified by the E. coli H-NS protein. We describe how transverse magnetic tweezers is a powerful tool that can be used to characterize the DNA binding and organization modes of NAPs and how such information leads to better understanding of its roles in DNA packaging of bacterial nucleoid and transcription regulation.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 20%
Researcher 2 20%
Student > Master 1 10%
Student > Doctoral Student 1 10%
Professor > Associate Professor 1 10%
Other 1 10%
Unknown 2 20%
Readers by discipline Count As %
Physics and Astronomy 2 20%
Biochemistry, Genetics and Molecular Biology 1 10%
Agricultural and Biological Sciences 1 10%
Neuroscience 1 10%
Unknown 5 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2018.
All research outputs
#17,945,904
of 23,043,346 outputs
Outputs from Methods in molecular biology
#7,317
of 13,194 outputs
Outputs of similar age
#294,602
of 421,409 outputs
Outputs of similar age from Methods in molecular biology
#641
of 1,074 outputs
Altmetric has tracked 23,043,346 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,194 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,409 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.

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