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The Bacterial Nucleoid

Overview of attention for book
Cover of 'The Bacterial Nucleoid'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli
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    Chapter 2 Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes
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    Chapter 3 Revealing Sister Chromatid Interactions with the loxP/ Cre Recombination Assay
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    Chapter 4 Transposon Insertion Site Sequencing for Synthetic Lethal Screening
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    Chapter 5 WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites
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    Chapter 6 High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing
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    Chapter 7 Generation and Analysis of Chromosomal Contact Maps of Bacteria
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    Chapter 8 Nucleoid-Associated Proteins: Genome Level Occupancy and Expression Analysis
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    Chapter 9 Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins
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    Chapter 10 A Chromosome Co-Entrapment Assay to Study Topological Protein–DNA Interactions
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    Chapter 11 Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins
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    Chapter 12 Biochemical Analysis of Bacterial Condensins
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    Chapter 13 Exploring Condensins with Magnetic Tweezers
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    Chapter 14 Applications of Magnetic Tweezers to Studies of NAPs
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    Chapter 15 A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data
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    Chapter 16 Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization
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    Chapter 17 Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage
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    Chapter 18 Measuring In Vivo Protein Dynamics Throughout the Cell Cycle Using Microfluidics
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    Chapter 19 Imaging of Bacterial Chromosome Organization by 3D Super-Resolution Microscopy
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    Chapter 20 Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins: The Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
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    Chapter 21 Procedures for Model-Guided Data Analysis of Chromosomal Loci Dynamics at Short Time Scales
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    Chapter 22 Isolation and Characterization of Bacterial Nucleoids in Microfluidic Devices
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    Chapter 23 Modeling Bacterial DNA: Simulation of Self-Avoiding Supercoiled Worm-Like Chains Including Structural Transitions of the Helix
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    Chapter 24 Molecular Dynamics Simulation of Supercoiled, Knotted, and Catenated DNA Molecules, Including Modeling of Action of DNA Gyrase
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    Chapter 25 Erratum to: Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins, the Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells
Attention for Chapter 16: Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization
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Chapter title
Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization
Chapter number 16
Book title
The Bacterial Nucleoid
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7098-8_16
Pubmed ID
Book ISBNs
978-1-4939-7097-1, 978-1-4939-7098-8
Authors

Visser, Bryan J., Joshi, Mohan C., Bates, David, Bryan J. Visser, Mohan C. Joshi, David Bates

Abstract

Fluorescence in situ hybridization (FISH) is a widely used technique to detect and localize specific DNA or RNA sequences in cells. Although supplanted in many ways by fluorescently labeled DNA binding proteins, FISH remains the only cytological method to examine many genetic loci at once (up to six), and can be performed in any cell type and genotype. These advantages have proved invaluable in studying the spatial relationships between chromosome regions and the dynamics of chromosome segregation in bacteria. A detailed protocol for DNA FISH in E. coli is described.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 31%
Student > Master 2 15%
Researcher 2 15%
Professor 1 8%
Unspecified 1 8%
Other 2 15%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 38%
Agricultural and Biological Sciences 2 15%
Unspecified 1 8%
Veterinary Science and Veterinary Medicine 1 8%
Computer Science 1 8%
Other 1 8%
Unknown 2 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2018.
All research outputs
#20,483,282
of 23,045,021 outputs
Outputs from Methods in molecular biology
#9,975
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Outputs of similar age
#356,342
of 421,413 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
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