| Chapter title |
Isolation and Characterization of Bacterial Nucleoids in Microfluidic Devices
|
|---|---|
| Chapter number | 22 |
| Book title |
The Bacterial Nucleoid
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7098-8_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7097-1, 978-1-4939-7098-8
|
| Authors |
James Pelletier, Suckjoon Jun, Pelletier, James, Jun, Suckjoon |
| Abstract |
We report methods for isolation of Escherichia coli nucleoids in microfluidic devices, allowing characterization of nucleoids during a controlled in vivo to in vitro transition. Biochemically, nucleoids are isolated by gentle osmotic lysis, which minimally perturbs nucleoid-associated proteins (NAPs). Biophysically, nucleoids are isolated in microfluidic chambers, which mimic confinement within the cell, as well as facilitate diffusive buffer exchange around nucleoids without subjecting them to flow. These methods can be used to characterize interactions between NAPs and whole nucleoids, and to investigate nucleoid structure and dynamics in confinement. We present protocols for isolation, quantification, and perturbation of nucleoids in microfluidic confinement. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 25% |
| Student > Bachelor | 2 | 17% |
| Student > Master | 2 | 17% |
| Student > Doctoral Student | 1 | 8% |
| Unspecified | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Physics and Astronomy | 2 | 17% |
| Unspecified | 1 | 8% |
| Chemistry | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 33% |