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Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle

Overview of attention for article published in Brazilian Journal of Microbiology, January 2014
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1 tweeter

Citations

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32 Mendeley
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Title
Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
Published in
Brazilian Journal of Microbiology, January 2014
DOI 10.1590/s1517-83822014005000039
Pubmed ID
Authors

Carlos A.N. Ramos, Flábio R. Araújo, Rafaelle C. Santos, Elaine S.P. Melo, Letícia C. Sousa, Carlos E.S. Vidal, Neurisvan R. Guerra, Rafael A.N. Ramos

Abstract

The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 3%
Colombia 1 3%
Unknown 30 94%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 22%
Researcher 6 19%
Student > Doctoral Student 5 16%
Other 2 6%
Student > Postgraduate 2 6%
Other 6 19%
Unknown 4 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 28%
Biochemistry, Genetics and Molecular Biology 5 16%
Veterinary Science and Veterinary Medicine 3 9%
Medicine and Dentistry 2 6%
Engineering 2 6%
Other 6 19%
Unknown 5 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2014.
All research outputs
#17,240,002
of 21,340,745 outputs
Outputs from Brazilian Journal of Microbiology
#393
of 544 outputs
Outputs of similar age
#143,611
of 202,797 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#3
of 3 outputs
Altmetric has tracked 21,340,745 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 544 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
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We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.