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Wheat Rust Diseases

Overview of attention for book
Cover of 'Wheat Rust Diseases'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping
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    Chapter 2 Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance
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    Chapter 3 Race Typing of Puccinia striiformis on Wheat
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    Chapter 4 Assessment of Aggressiveness of Puccinia striiformis on Wheat
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    Chapter 5 Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing
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    Chapter 6 Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis
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    Chapter 7 Computational Methods for Predicting Effectors in Rust Pathogens
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    Chapter 8 Protein–Protein Interaction Assays with Effector–GFP Fusions in Nicotiana benthamiana
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    Chapter 9 Proteome Profiling by 2D–Liquid Chromatography Method for Wheat–Rust Interaction
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    Chapter 10 Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing
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    Chapter 11 Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi
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    Chapter 12 Genetic Analysis of Resistance to Wheat Rusts
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    Chapter 13 Advances in Identification and Mapping of Rust Resistance Genes in Wheat
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    Chapter 14 Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives
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    Chapter 15 Applications of Genomic Selection in Breeding Wheat for Rust Resistance
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    Chapter 16 Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown under Controlled Conditions
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    Chapter 17 Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene Cloning
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    Chapter 18 Isolation of Wheat Genomic DNA for Gene Mapping and Cloning
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    Chapter 19 MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
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    Chapter 20 Rapid Gene Isolation Using MutChromSeq
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    Chapter 21 Rapid Identification of Rust Resistance Genes Through Cultivar-Specific De Novo Chromosome Assemblies
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    Chapter 22 BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance
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    Chapter 23 Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene that Encodes a Hexose Transporter
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    Chapter 24 Biocontrol Agents for Controlling Wheat Rust
Attention for Chapter 17: Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene Cloning
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Chapter title
Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene Cloning
Chapter number 17
Book title
Wheat Rust Diseases
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7249-4_17
Pubmed ID
Book ISBNs
978-1-4939-7248-7, 978-1-4939-7249-4
Authors

Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B. H. Wulff, Evans Lagudah

Abstract

One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the isolation, identification and cloning of rust resistance genes. In this chapter we describe a protocol for seed mutagenesis of wheat with EMS and sodium azide.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 21 66%
Student > Ph. D. Student 3 9%
Researcher 3 9%
Professor > Associate Professor 1 3%
Unknown 4 13%
Readers by discipline Count As %
Unspecified 21 66%
Agricultural and Biological Sciences 5 16%
Biochemistry, Genetics and Molecular Biology 2 6%
Unknown 4 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2017.
All research outputs
#20,444,703
of 22,999,744 outputs
Outputs from Methods in molecular biology
#9,934
of 13,154 outputs
Outputs of similar age
#356,140
of 421,208 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
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So far Altmetric has tracked 13,154 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.