| Chapter title |
Rapid Identification of Rust Resistance Genes Through Cultivar-Specific De Novo Chromosome Assemblies
|
|---|---|
| Chapter number | 21 |
| Book title |
Wheat Rust Diseases
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7249-4_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7248-7, 978-1-4939-7249-4
|
| Authors |
Anupriya K. Thind, Thomas Wicker, Simon G. Krattinger |
| Abstract |
"Map-based cloning" is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100-200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 50% |
| Researcher | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |
| Agricultural and Biological Sciences | 1 | 50% |