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Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus

Overview of attention for article published in Virology Journal, September 2017
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Title
Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus
Published in
Virology Journal, September 2017
DOI 10.1186/s12985-017-0838-x
Pubmed ID
Authors

Anna Stachyra, Monika Rak, Patrycja Redkiewicz, Zbigniew Madeja, Katarzyna Gawarecka, Tadeusz Chojnacki, Ewa Świeżewska, Marek Masnyk, Marek Chmielewski, Agnieszka Sirko, Anna Góra-Sochacka

Abstract

Cationic derivatives of polyprenols (trimethylpolyprenylammonium iodides - PTAI) with variable chain length between 6 and 15 isoprene units prepared from naturally occurring poly-cis-prenols were tested as DNA vaccine carriers in chickens and mice. This study aimed to investigate if PTAI could be used as an efficient carrier of a DNA vaccine. Several vaccine mixtures were prepared by combining different proportions of the vaccine plasmid (carrying cDNA encoding a vaccine antigen, hemagglutinin from H5N1 influenza virus) and various compositions of PTAI. The vaccines were delivered by intramuscular injection to either chickens or mice. The presence of specific antibodies in sera collected from the immunized animals was analyzed by enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) test. The mixtures of PTAI with helper lipids, such as DOPE (1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine), DC-cholesterol [{3ß-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol} hydrochloride] or DOPC (1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) induced strong humoral response to the antigen encoded by the DNA vaccine plasmid. The animal immunization results confirmed that PTAI compositions, especially mixtures of PTAI with DOPE and DC-cholesterol, do work as effective carriers of DNA vaccines, comparable to the commercially available lipid transfection reagent.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 25%
Student > Ph. D. Student 3 19%
Student > Bachelor 2 13%
Student > Doctoral Student 1 6%
Professor > Associate Professor 1 6%
Other 1 6%
Unknown 4 25%
Readers by discipline Count As %
Medicine and Dentistry 3 19%
Pharmacology, Toxicology and Pharmaceutical Science 2 13%
Immunology and Microbiology 1 6%
Biochemistry, Genetics and Molecular Biology 1 6%
Social Sciences 1 6%
Other 1 6%
Unknown 7 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2017.
All research outputs
#18,571,001
of 23,001,641 outputs
Outputs from Virology Journal
#2,453
of 3,058 outputs
Outputs of similar age
#242,504
of 316,396 outputs
Outputs of similar age from Virology Journal
#47
of 57 outputs
Altmetric has tracked 23,001,641 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,058 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.8. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,396 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one is in the 3rd percentile – i.e., 3% of its contemporaries scored the same or lower than it.