Title |
Development and validation of a new high-throughput method to investigate the clonality of HTLV-1-infected cells based on provirus integration sites
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Published in |
Genome Medicine, June 2014
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DOI | 10.1186/gm568 |
Pubmed ID | |
Authors |
Sanaz Firouzi, Yosvany López, Yutaka Suzuki, Kenta Nakai, Sumio Sugano, Tadanori Yamochi, Toshiki Watanabe |
Abstract |
Transformation and clonal proliferation of T-cells infected with human T-cell leukemia virus type-I (HTLV-1) cause adult T-cell leukemia. We took advantage of next-generation sequencing technology to develop and internally validate a new methodology for isolating integration sites and estimating the number of cells in each HTLV-1-infected clone (clone size). Initial analysis was performed with DNA samples from infected individuals. We then used appropriate controls with known integration sites and clonality status to confirm the accuracy of our system, which indeed had the least errors among the currently available techniques. Results suggest potential clinical and biological applications of the new method. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Unknown | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Italy | 2 | 3% |
Germany | 1 | 1% |
Brazil | 1 | 1% |
United Kingdom | 1 | 1% |
Denmark | 1 | 1% |
Unknown | 63 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 20 | 29% |
Student > Ph. D. Student | 19 | 28% |
Student > Bachelor | 6 | 9% |
Student > Master | 5 | 7% |
Student > Doctoral Student | 3 | 4% |
Other | 9 | 13% |
Unknown | 7 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 35% |
Biochemistry, Genetics and Molecular Biology | 15 | 22% |
Medicine and Dentistry | 10 | 14% |
Immunology and Microbiology | 3 | 4% |
Engineering | 3 | 4% |
Other | 6 | 9% |
Unknown | 8 | 12% |