Genome sequence of the lupin-nodulating Bradyrhizobium sp. strain WSM1417

Wayne Reeve, Jason Terpolilli, Vanessa Melino, Julie Ardley, Rui Tian, Sofie De Meyer, Ravi Tiwari, Ronald Yates, Graham O’Hara, John Howieson, Mohamed Ninawi, Hazuki Teshima, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavrommatis, Victor Markowitz, Natalia Ivanova, Galina Ovchinnikova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Lin Peters, Tanja Woyke, Nikos Kyrpides

Bradyrhizobium sp. strain WSM1417 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen (N2) fixing root nodule of Lupinus sp. collected in Papudo, Chile, in 1995. However, this microsymbiont is a poorly effective N2 fixer with the legume host Lupinus angustifolius L.; a lupin species of considerable economic importance in both Chile and Australia. The symbiosis formed with L. angustifolius produces less than half of the dry matter achieved by the symbioses with commercial inoculant strains such as Bradyrhizobium sp. strain WSM471. Therefore, WSM1417 is an important candidate strain with which to investigate the genetics of effective N2 fixation in the lupin-bradyrhizobia symbioses. Here we describe the features of Bradyrhizobium sp. strain WSM1417, together with genome sequence information and annotation. The 8,048,963 bp high-quality-draft genome is arranged in a single scaffold of 2 contigs, contains 7,695 protein-coding genes and 77 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.