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Gene transcription in sea otters (Enhydra lutris); development of a diagnostic tool for sea otter and ecosystem health

Overview of attention for article published in Molecular Ecology Resources, August 2011
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#36 of 416)
  • High Attention Score compared to outputs of the same age (92nd percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

news
1 news outlet
twitter
1 tweeter

Readers on

mendeley
24 Mendeley
connotea
1 Connotea
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Title
Gene transcription in sea otters (Enhydra lutris); development of a diagnostic tool for sea otter and ecosystem health
Published in
Molecular Ecology Resources, August 2011
DOI 10.1111/j.1755-0998.2011.03060.x
Pubmed ID
Authors

BOWEN, LIZABETH, MILES, A. KEITH, MURRAY, MICHAEL, HAULENA, MARTIN, TUTTLE, JUDY, VAN BONN, WILLIAM, ADAMS, LANCE, BODKIN, JAMES L., BALLACHEY, BRENDA, ESTES, JAMES, TINKER, M. TIM, KEISTER, ROBIN, STOTT, JEFFREY L., Bowen L, Miles AK, Murray M, Haulena M, Tuttle J, VAN Bonn W, Adams L, Bodkin JL, Ballachey B, Estes J, Tinker MT, Keister R, Stott JL

Abstract

Gene transcription analysis for diagnosing or monitoring wildlife health requires the ability to distinguish pathophysiological change from natural variation. Herein, we describe methodology for the development of quantitative real-time polymerase chain reaction (qPCR) assays to measure differential transcript levels of multiple immune function genes in the sea otter (Enhydra lutris); sea otter-specific qPCR primer sequences for the genes of interest are defined. We establish a 'reference' range of transcripts for each gene in a group of clinically healthy captive and free-ranging sea otters. The 10 genes of interest represent multiple physiological systems that play a role in immuno-modulation, inflammation, cell protection, tumour suppression, cellular stress response, xenobiotic metabolizing enzymes, antioxidant enzymes and cell-cell adhesion. The cycle threshold (C(T)) measures for most genes were normally distributed; the complement cytolysis inhibitor was the exception. The relative enumeration of multiple gene transcripts in simple peripheral blood samples expands the diagnostic capability currently available to assess the health of sea otters in situ and provides a better understanding of the state of their environment.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 29%
Student > Ph. D. Student 6 25%
Student > Bachelor 4 17%
Student > Master 4 17%
Other 2 8%
Other 1 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 83%
Veterinary Science and Veterinary Medicine 1 4%
Environmental Science 1 4%
Earth and Planetary Sciences 1 4%
Medicine and Dentistry 1 4%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2012.
All research outputs
#292,097
of 4,507,072 outputs
Outputs from Molecular Ecology Resources
#36
of 416 outputs
Outputs of similar age
#4,712
of 62,267 outputs
Outputs of similar age from Molecular Ecology Resources
#1
of 5 outputs
Altmetric has tracked 4,507,072 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 416 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 62,267 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them