Chapter title |
Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery
|
---|---|
Chapter number | 6 |
Book title |
Proteomics
|
Published in |
Methods in molecular biology, January 2009
|
DOI | 10.1007/978-1-60761-157-8_6 |
Pubmed ID | |
Book ISBNs |
978-1-60761-156-1, 978-1-60761-157-8
|
Authors |
Petra Zürbig, Eric Schiffer, Harald Mischak, Zürbig, Petra, Schiffer, Eric, Mischak, Harald |
Abstract |
Currently, the main focus of clinical proteome analysis is on detection and identification of polypeptides that significantly change owing to pathological changes. Capillary electrophoresis coupled online to an electrospray ionization time of flight mass spectrometer (CE-MS) allows the differential display of a large number of polypeptides in a single, reproducible, and time-limited step and enables the comparison of different protein profiles for biomarker discovery. In addition to the reproducibility of the CE-MS setup, many further steps including data processing and mining, usage of biomarkers for diagnosis, and biomarker sequencing are necessary to answer the demands of biomarker discovery of clinical significance. In this chapter, we discuss materials and methods for CE-MS-based clinical proteomics allowing the reproducible profiling of urine. |
X Demographics
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United States | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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South Africa | 1 | 5% |
Unknown | 19 | 95% |
Demographic breakdown
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Student > Ph. D. Student | 6 | 30% |
Researcher | 4 | 20% |
Professor | 3 | 15% |
Other | 2 | 10% |
Student > Doctoral Student | 1 | 5% |
Other | 2 | 10% |
Unknown | 2 | 10% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 35% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Computer Science | 1 | 5% |
Chemistry | 1 | 5% |
Other | 0 | 0% |
Unknown | 2 | 10% |