Chapter title |
Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory Fluxes
|
---|---|
Chapter number | 2 |
Book title |
Plant Respiration and Internal Oxygen
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7292-0_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7291-3, 978-1-4939-7292-0
|
Authors |
Toshihiro Obata, Laise Rosado-Souza, Alisdair R. Fernie, Obata, Toshihiro, Rosado-Souza, Laise, Fernie, Alisdair R. |
Abstract |
Carbohydrates catabolized via respiratory processes are not only used for energy production but also for biosynthesis of cellular components including soluble molecules (sugars, amino acids, organic acids, and their derivatives) and insoluble macromolecules (proteins, starch, and cell wall). Radiotracer experiments using (14)C-labeled glucose provide a global picture of the fate of respired carbon in the metabolic network. This method is based on a chemical fractionation of biomolecules in (14)C-glucose fed plant materials and the subsequent determination of radioactivity in each fraction. Metabolic flux into each fraction can be estimated from the specific activity of the hexose phosphate pool. Here, we describe the procedure for glucose metabolism in potato tuber but similar protocols can be adopted for various plant organs and substrates. |
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