| Chapter title |
Isolation and Structural Studies of Mitochondria from Pea Roots
|
|---|---|
| Chapter number | 10 |
| Book title |
Plant Respiration and Internal Oxygen
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7292-0_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7291-3, 978-1-4939-7292-0
|
| Authors |
Abhaypratap Vishwakarma, Kapuganti Jagadis Gupta |
| Abstract |
For structural and respiratory studies, isolation of intact and active mitochondria is essential. Here, we describe an isolation method which gave good yield and intact mitochondria from 2-week-old pea (Pisum sativum) roots grown hydroponically under standard growth conditions. We used Percoll gradient centrifugation for this isolation procedure. The yield of purified mitochondria was 50 μg/g FW. Isolated mitochondria maintained their structure which was observed by using MitoTracker green in confocal microscope and scanning electron microscopy (SEM). Intact mitochondria are clearly visible in SCM images. Taken together this isolation method can be used for physiological and microscopic studies on mitochondria. |
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