| Chapter title |
A Method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution
|
|---|---|
| Chapter number | 3 |
| Book title |
Plant Respiration and Internal Oxygen
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7292-0_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7291-3, 978-1-4939-7292-0
|
| Authors |
Hardy Rolletschek, Gregor Liebsch |
| Abstract |
Conventional oxygen (micro-) sensors assess oxygen concentration within a particular region or across a transect of tissue, but provide no information regarding its bidimensional distribution. Here, a novel imaging technology is presented, in which an optical sensor foil (i.e., the planar optode) is attached to the surface of the sample. The sensor converts a fluorescent signal into an oxygen value. Since each single image captures an entire area of the sample surface, the system is able to deduce the distribution of oxygen at a resolution level of few micrometers. It can be deployed to dynamically monitor oxygen consumption, thereby providing a detailed respiration map at close to cellular resolution. Here, we demonstrate the application of the imaging tool to developing plant seeds; the protocol is explained step by step and some potential pitfalls are discussed. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 32% |
| Student > Ph. D. Student | 3 | 14% |
| Student > Master | 3 | 14% |
| Other | 2 | 9% |
| Unspecified | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 4 | 18% |
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Medicine and Dentistry | 3 | 14% |
| Agricultural and Biological Sciences | 2 | 9% |
| Earth and Planetary Sciences | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 6 | 27% |