| Chapter title |
Measurement of Mitochondrial Respiration in Isolated Protoplasts: Cytochrome and Alternative Pathways
|
|---|---|
| Chapter number | 22 |
| Book title |
Plant Respiration and Internal Oxygen
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7292-0_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7291-3, 978-1-4939-7292-0
|
| Authors |
Bobba Sunil, Agepati S. Raghavendra |
| Abstract |
The electron partitioning between COX and AOX pathways of mitochondria and their coordination is necessary to meet the energy demands as well as to maintain optimized redox status in plants under varying environmental conditions. The relative contribution of these two pathways to total respiration is an important measure during a given stress condition. We describe in detail the procedure that allows the measurement of the parameters of COX and AOX pathway of respiration in mesophyll protoplasts using Clark-type O2 electrode. This chapter also lists the steps for rapid isolation procedure for mesophyll protoplasts from pea leaves. The advantages and limitations of the use of metabolic inhibitors and the protoplasts for measuring the respiration are also briefly discussed. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Researcher | 3 | 30% |
| Professor | 1 | 10% |
| Student > Master | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 60% |
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Unknown | 1 | 10% |