Title |
Stable nuclear transformation of Pandorina morum
|
---|---|
Published in |
BMC Biotechnology, July 2014
|
DOI | 10.1186/1472-6750-14-65 |
Pubmed ID | |
Authors |
Kai Lerche, Armin Hallmann |
Abstract |
Volvocine green algae like Pandorina morum represent one of the most recent inventions of multicellularity diverged from their unicellular relatives. The 8-16 celled P. morum alga and its close multicellular relatives constitute a model lineage for research into cellular differentiation, morphogenesis and epithelial folding, sexual reproduction and evolution of multicellularity. Pandorina is the largest and most complex organism in the volvocine lineage that still exhibits isogamous sexual reproduction. So far, molecular-biological investigations in P. morum were constricted due to the absence of methods for transformation of this species, which is a prerequisite for introduction of reporter genes and (modified) genes of interest. |
X Demographics
Geographical breakdown
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Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 50% |
Scientists | 1 | 25% |
Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 5% |
Unknown | 41 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 13 | 30% |
Researcher | 7 | 16% |
Professor | 4 | 9% |
Student > Bachelor | 2 | 5% |
Student > Doctoral Student | 2 | 5% |
Other | 8 | 19% |
Unknown | 7 | 16% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 16 | 37% |
Biochemistry, Genetics and Molecular Biology | 9 | 21% |
Social Sciences | 3 | 7% |
Environmental Science | 2 | 5% |
Business, Management and Accounting | 1 | 2% |
Other | 2 | 5% |
Unknown | 10 | 23% |