Chapter title |
Identification and Quantification of Cyclic Di-Guanosine Monophosphate and Its Linear Metabolites by Reversed-Phase LC-MS/MS.
|
---|---|
Chapter number | 5 |
Book title |
c-di-GMP Signaling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7240-1_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7239-5, 978-1-4939-7240-1
|
Authors |
Bähre, Heike, Kaever, Volkhard, Heike Bähre, Volkhard Kaever |
Abstract |
Cyclic dinucleotides such as bis-(3',5')-cyclic dimeric guanosine monophosphate (3',3'-c-di-GMP) represent an important class of second messengers in bacteria and are involved in numerous (patho)physiological settings. Here, we describe a sensitive and specific quantification method for 3',3'-c-di-GMP by HPLC-coupled tandem mass spectrometry (LC-MS/MS). Additionally, linear 3',3'-c-di-GMP metabolites, i.e., 5'-phosphoguanylyl-3',5'-guanosine (pGpG) and 5'-guanosine monophosphate (5'-GMP), as well as cyclic guanosine monophosphate (3',5'-cGMP) and 3',3' c-di-GMP analogues (2',3'-c-di-GMP and 2',2'-c-di-GMP) can be simultaneously determined by this method. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 24% |
Student > Master | 3 | 14% |
Student > Doctoral Student | 2 | 10% |
Lecturer | 1 | 5% |
Student > Ph. D. Student | 1 | 5% |
Other | 3 | 14% |
Unknown | 6 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 24% |
Agricultural and Biological Sciences | 3 | 14% |
Immunology and Microbiology | 2 | 10% |
Medicine and Dentistry | 2 | 10% |
Unspecified | 1 | 5% |
Other | 1 | 5% |
Unknown | 7 | 33% |