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Mendeley readers
Chapter title |
Identification of c-di-AMP-Binding Proteins Using Magnetic Beads
|
---|---|
Chapter number | 27 |
Book title |
c-di-GMP Signaling
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7240-1_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7239-5, 978-1-4939-7240-1
|
Authors |
Jan Kampf, Jan Gundlach, Christina Herzberg, Katrin Treffon, Jörg Stülke |
Abstract |
To identify cytosolic proteins that bind to cyclic di-AMP, a biotinylated analog of the nucleotide is used for protein pull-down experiments. In this approach, biotinylated c-di-AMP is coupled to Streptactin-covered beads. After protein separation using standard SDS-PAGE, the protein(s) of interest are identified by mass spectrometric analyses. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 38% |
Student > Master | 3 | 23% |
Professor | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 54% |
Agricultural and Biological Sciences | 2 | 15% |
Chemistry | 2 | 15% |
Unknown | 2 | 15% |