Chapter title |
Using Light-Activated Enzymes for Modulating Intracellular c-di-GMP Levels in Bacteria
|
---|---|
Chapter number | 14 |
Book title |
c-di-GMP Signaling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7240-1_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7239-5, 978-1-4939-7240-1
|
Authors |
Min-Hyung Ryu, Anastasia Fomicheva, Lindsey O’Neal, Gladys Alexandre, Mark Gomelsky |
Abstract |
Signaling pathways involving second messenger c-di-GMP regulate various aspects of bacterial physiology and behavior. We describe the use of a red light-activated diguanylate cyclase (c-di-GMP synthase) and a blue light-activated c-di-GMP phosphodiesterase (hydrolase) for manipulating intracellular c-di-GMP levels in bacterial cells. We illustrate the application of these enzymes in regulating several c-di-GMP-dependent phenotypes, i.e., motility and biofilm phenotypes in E. coli and chemotactic behavior in the alphaproteobacterium Azospirillum brasilense. We expect these light-activated enzymes to be also useful in regulating c-di-GMP-dependent processes occurring at the fast timescale, for spatial control of bacterial populations, as well as for analyzing c-di-GMP-dependent phenomena at the single-cell level. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 21% |
Student > Bachelor | 4 | 17% |
Student > Ph. D. Student | 4 | 17% |
Professor > Associate Professor | 3 | 13% |
Student > Master | 2 | 8% |
Other | 2 | 8% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 3 | 13% |
Immunology and Microbiology | 2 | 8% |
Other | 1 | 4% |
Unknown | 4 | 17% |