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X Demographics
Mendeley readers
Attention Score in Context
| Title |
RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection
|
|---|---|
| Published in |
Proceedings of the National Academy of Sciences of the United States of America, July 2014
|
| DOI | 10.1073/pnas.1405186111 |
| Pubmed ID | |
| Authors |
Wenhui Hu, Rafal Kaminski, Fan Yang, Yonggang Zhang, Laura Cosentino, Fang Li, Biao Luo, David Alvarez-Carbonell, Yoelvis Garcia-Mesa, Jonathan Karn, Xianming Mo, Kamel Khalili |
| Abstract |
AIDS remains incurable due to the permanent integration of HIV-1 into the host genome, imparting risk of viral reactivation even after antiretroviral therapy. New strategies are needed to ablate the viral genome from latently infected cells, because current methods are too inefficient and prone to adverse off-target effects. To eliminate the integrated HIV-1 genome, we used the Cas9/guide RNA (gRNA) system, in single and multiplex configurations. We identified highly specific targets within the HIV-1 LTR U3 region that were efficiently edited by Cas9/gRNA, inactivating viral gene expression and replication in latently infected microglial, promonocytic, and T cells. Cas9/gRNAs caused neither genotoxicity nor off-target editing to the host cells, and completely excised a 9,709-bp fragment of integrated proviral DNA that spanned from its 5' to 3' LTRs. Furthermore, the presence of multiplex gRNAs within Cas9-expressing cells prevented HIV-1 infection. Our results suggest that Cas9/gRNA can be engineered to provide a specific, efficacious prophylactic and therapeutic approach against AIDS. |
X Demographics
The data shown below were collected from the profiles of 221 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 39 | 18% |
| Mexico | 29 | 13% |
| United Kingdom | 13 | 6% |
| Spain | 10 | 5% |
| Germany | 5 | 2% |
| France | 3 | 1% |
| South Africa | 2 | <1% |
| Oman | 2 | <1% |
| Canada | 2 | <1% |
| Other | 20 | 9% |
| Unknown | 96 | 43% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 174 | 79% |
| Scientists | 38 | 17% |
| Science communicators (journalists, bloggers, editors) | 5 | 2% |
| Practitioners (doctors, other healthcare professionals) | 4 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 908 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 23 | 3% |
| Germany | 5 | <1% |
| United Kingdom | 4 | <1% |
| Japan | 4 | <1% |
| Canada | 3 | <1% |
| France | 2 | <1% |
| South Africa | 2 | <1% |
| Spain | 2 | <1% |
| Mexico | 2 | <1% |
| Other | 7 | <1% |
| Unknown | 854 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 184 | 20% |
| Student > Ph. D. Student | 178 | 20% |
| Student > Master | 139 | 15% |
| Researcher | 126 | 14% |
| Student > Doctoral Student | 32 | 4% |
| Other | 110 | 12% |
| Unknown | 139 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 316 | 35% |
| Biochemistry, Genetics and Molecular Biology | 201 | 22% |
| Medicine and Dentistry | 71 | 8% |
| Immunology and Microbiology | 58 | 6% |
| Chemistry | 20 | 2% |
| Other | 80 | 9% |
| Unknown | 162 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 806. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 April 2024.
All research outputs
#23,895
of 25,870,142 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#725
of 103,885 outputs
Outputs of similar age
#134
of 240,669 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#11
of 916 outputs
Altmetric has tracked 25,870,142 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 103,885 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 39.5. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,669 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 916 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.