Title |
COUP-TFII regulates metastasis of colorectal adenocarcinoma cells by modulating Snail1
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Published in |
British Journal of Cancer, July 2014
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DOI | 10.1038/bjc.2014.373 |
Pubmed ID | |
Authors |
Y Bao, D Gu, W Feng, X Sun, X Wang, X Zhang, Q Shi, G Cui, H Yu, C Tang, A Deng |
Abstract |
Background:Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII, also known as NR2F2) promotes metastasis by functioning in the tumour microenvironment; however, the role of COUP-TFII in colorectal cancer remains unknown.Methods:Human colon adenocarcinoma tissues were collected to test COUP-TFII expression. Wound-healing and cell invasion assay were used to evaluate migration and invasion of cells. Chicken ovalbumin upstream promoter-transcription factor II and related protein expression was assessed by immunostaining, immunoblotting and real-time PCR assay. Tamoxifen-inducible COUP-TFII knockout mice were employed to test COUP-TFII functions on colon cancer metastasis in vivo.Results:Elevated expression of COUP-TFII in colorectal adenocarcinoma tissue correlated with overexpression of the Snail1 transcription factor. High COUP-TFII expression correlated with metastasis and shorter patient survival. Chicken ovalbumin upstream promoter-transcription factor II regulated the migration and invasion of cancer cells. With Snail1, COUP-TFII inhibited expression of adherence molecules such as ZO-1, E-cadherin and β-catenin in colorectal cancer cells. Overexpression of COUP-TFII was required for cancer cells to metastasise in vivo. Chicken ovalbumin upstream promoter-transcription factor II regulated the transcription and expression of Snail1 by directly targeting the Snail1 promoter and regulated associated genes.Conclusions:Chicken ovalbumin upstream promoter-transcription factor II was crucial for colorectal cancer metastasis and regulated cell migration and metastasis in conjunction with Snail1. Chicken ovalbumin upstream promoter-transcription factor II was found to be a biomarker associated with patient survival and colorectal cancer metastasis.British Journal of Cancer advance online publication, 17 July 2014; doi:10.1038/bjc.2014.373 www.bjcancer.com. |
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Demographic breakdown
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Mendeley readers
Geographical breakdown
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Demographic breakdown
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Student > Ph. D. Student | 5 | 24% |
Student > Doctoral Student | 3 | 14% |
Researcher | 3 | 14% |
Unspecified | 2 | 10% |
Student > Master | 2 | 10% |
Other | 3 | 14% |
Unknown | 3 | 14% |
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Medicine and Dentistry | 4 | 19% |
Biochemistry, Genetics and Molecular Biology | 4 | 19% |
Unspecified | 2 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Other | 1 | 5% |
Unknown | 3 | 14% |