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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Circumventing qPCR inhibition to amplify miRNAs in plasma
|
|---|---|
| Published in |
Biomarker Research, July 2014
|
| DOI | 10.1186/2050-7771-2-13 |
| Pubmed ID | |
| Abstract |
Circulating microRNAs (c-miRNAs) have be identified in saliva, urine and blood, which has led to increasing interest in their development as biomarkers for diverse diseases including cancers. One of the key advantages of c-miRNAs over other biomarkers is the ability to be amplified and quantified by quantitative PCR (qPCR). However, at phlebotomy when whole blood is dispensed into heparinized tubes, residual levels of the anti-coagulant lithium heparin may remain in the plasma and hence with RNA isolated from the plasma. This can confound the detection of c-miRNAs by qPCR because it inhibits reverse transcriptase (RT). Here we present a procedure, modified from earlier techniques, to detect c-miRNAs in plasma that improves sensitivity and streamlines performance. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | 2% |
| Unknown | 53 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 20% |
| Student > Ph. D. Student | 8 | 15% |
| Student > Master | 8 | 15% |
| Other | 5 | 9% |
| Student > Bachelor | 4 | 7% |
| Other | 6 | 11% |
| Unknown | 12 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 28% |
| Biochemistry, Genetics and Molecular Biology | 11 | 20% |
| Medicine and Dentistry | 9 | 17% |
| Immunology and Microbiology | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 13 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 August 2014.
All research outputs
#15,303,385
of 22,759,618 outputs
Outputs from Biomarker Research
#157
of 310 outputs
Outputs of similar age
#132,208
of 228,540 outputs
Outputs of similar age from Biomarker Research
#2
of 2 outputs
Altmetric has tracked 22,759,618 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 310 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,540 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.