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Effect of simulated gastro-duodenal digestion on the allergenic reactivity of beta-lactoglobulin

Overview of attention for article published in Clinical and Translational Allergy, January 2011
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Title
Effect of simulated gastro-duodenal digestion on the allergenic reactivity of beta-lactoglobulin
Published in
Clinical and Translational Allergy, January 2011
DOI 10.1186/2045-7022-1-6
Pubmed ID
Authors

Apostolos Bossios, Maria Theodoropoulou, Lucie Mondoulet, Neil M Rigby, Nikolaos G Papadopoulos, Hervé Bernard, Karine Adel-Patient, Jean-Michel Wal, Clare EN Mills, Photini Papageorgiou

Abstract

Cow's milk (CM) allergy affects about 2% of infants. The allergenicity of dietary proteins, including those from CM, has been related to their digestibility although the generality of the link and its causality remains to be demonstrated. In this study we use an in vitro digestion system, to investigate the digestibility of β-lactoglobulin (blg) during gastrointestinal transit and to assess the impact of this process on blg allergenic reactivity in CM allergic children. Blg digesta were prepared using an in vitro digestion protocol simulating either gastric digestion alone or followed by duodenal digestion with or without phosphatidylcholine (PC). Biochemical analysis of blg digesta was performed by SDS-PAGE and their concentration was measured by a sandwich ELISA. Assessment of their allergenic reactivity was done in vitro by EAST inhibition, specific basophil activation (basotest) and lymphocyte proliferation (PCNA-flow cytometry) assays using sera and cells from patients allergic to blg and in vivo by skin prick testing (SPT) of these patients. Blg was only broken down to smaller peptides after gastro-duodenal digestion although a sizeable amount of intact protein still remained. Digestion did not modify the IgE binding capacity of blg except for gastro-duodenal digestion performed in the absence of PC. These results are consistent with the quantity of intact blg remaining in the digesta. Overall both gastric and gastroduodenal digestion enhanced activation of sensitized basophils and proliferation of sensitized lymphocytes by blg. However, there was a tendency towards reduction in mean diameter of SPT following digestion, the PC alone during phase 1 digestion causing a significant increase in mean diameter. Digestion did not reduce the allergenic reactivity of blg to a clinically insignificant extent, PC inhibiting digestion and thereby protecting blg allergenic reactivity. SPT reactivity was reduced compared to blg immunoreactivity in in vitro tests.

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 2%
Unknown 51 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 19%
Student > Ph. D. Student 9 17%
Student > Master 7 13%
Other 5 10%
Professor > Associate Professor 5 10%
Other 10 19%
Unknown 6 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 35%
Medicine and Dentistry 6 12%
Chemistry 4 8%
Biochemistry, Genetics and Molecular Biology 4 8%
Immunology and Microbiology 3 6%
Other 9 17%
Unknown 8 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2011.
All research outputs
#3,921,587
of 4,681,163 outputs
Outputs from Clinical and Translational Allergy
#185
of 206 outputs
Outputs of similar age
#54,218
of 63,597 outputs
Outputs of similar age from Clinical and Translational Allergy
#9
of 9 outputs
Altmetric has tracked 4,681,163 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 206 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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