Title |
BESST - Efficient scaffolding of large fragmented assemblies
|
---|---|
Published in |
BMC Bioinformatics, August 2014
|
DOI | 10.1186/1471-2105-15-281 |
Pubmed ID | |
Authors |
Kristoffer Sahlin, Francesco Vezzi, Björn Nystedt, Joakim Lundeberg, Lars Arvestad |
Abstract |
The use of short reads from High Throughput Sequencing (HTS) techniques is now commonplace in de novo assembly. Yet, obtaining contiguous assemblies from short reads is challenging, thus making scaffolding an important step in the assembly pipeline. Different algorithms have been proposed but many of them use the number of read pairs supporting a linking of two contigs as an indicator of reliability. This reasoning is intuitive, but fails to account for variation in link count due to contig features.We have also noted that published scaffolders are only evaluated on small datasets using output from only one assembler. Two issues arise from this. Firstly, some of the available tools are not well suited for complex genomes. Secondly, these evaluations provide little support for inferring a software's general performance. |
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United Kingdom | 4 | 12% |
Sweden | 3 | 9% |
India | 2 | 6% |
Germany | 2 | 6% |
Switzerland | 1 | 3% |
France | 1 | 3% |
Canada | 1 | 3% |
Australia | 1 | 3% |
Other | 3 | 9% |
Unknown | 8 | 24% |
Demographic breakdown
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Scientists | 20 | 59% |
Members of the public | 13 | 38% |
Practitioners (doctors, other healthcare professionals) | 1 | 3% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 3 | 2% |
Sweden | 2 | 1% |
Norway | 1 | <1% |
Italy | 1 | <1% |
Netherlands | 1 | <1% |
Vietnam | 1 | <1% |
United Kingdom | 1 | <1% |
Poland | 1 | <1% |
Other | 0 | 0% |
Unknown | 143 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 51 | 32% |
Student > Ph. D. Student | 37 | 23% |
Student > Master | 15 | 9% |
Student > Bachelor | 10 | 6% |
Professor > Associate Professor | 9 | 6% |
Other | 21 | 13% |
Unknown | 15 | 9% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 28 | 18% |
Computer Science | 14 | 9% |
Engineering | 2 | 1% |
Immunology and Microbiology | 2 | 1% |
Other | 3 | 2% |
Unknown | 21 | 13% |