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Genetic and molecular identification of three human TPP1 functions in telomerase action: recruitment, activation, and homeostasis set point regulation

Overview of attention for article published in Genes & Development, August 2014
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (81st percentile)

Mentioned by

blogs
1 blog
wikipedia
1 Wikipedia page
f1000
1 research highlight platform

Citations

dimensions_citation
66 Dimensions

Readers on

mendeley
97 Mendeley
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Title
Genetic and molecular identification of three human TPP1 functions in telomerase action: recruitment, activation, and homeostasis set point regulation
Published in
Genes & Development, August 2014
DOI 10.1101/gad.246819.114
Pubmed ID
Authors

Alec N. Sexton, Samuel G. Regalado, Christine S. Lai, Gregory J. Cost, Colleen M. O’Neil, Fyodor D. Urnov, Philip D. Gregory, Rudolf Jaenisch, Kathleen Collins, Dirk Hockemeyer

Abstract

Telomere length homeostasis is essential for the long-term survival of stem cells, and its set point determines the proliferative capacity of differentiated cell lineages by restricting the reservoir of telomeric repeats. Knockdown and overexpression studies in human tumor cells showed that the shelterin subunit TPP1 recruits telomerase to telomeres through a region termed the TEL patch. However, these studies do not resolve whether the TPP1 TEL patch is the only mechanism for telomerase recruitment and whether telomerase regulation studied in tumor cells is representative of nontransformed cells such as stem cells. Using genome engineering of human embryonic stem cells, which have physiological telomere length homeostasis, we establish that the TPP1 TEL patch is genetically essential for telomere elongation and thus long-term cell viability. Furthermore, genetic bypass, protein fusion, and intragenic complementation assays define two distinct additional mechanisms of TPP1 involvement in telomerase action at telomeres. We demonstrate that TPP1 provides an essential step of telomerase activation as well as feedback regulation of telomerase by telomere length, which is necessary to determine the appropriate telomere length set point in human embryonic stem cells. These studies reveal and resolve multiple TPP1 roles in telomere elongation and stem cell telomere length homeostasis.

Mendeley readers

The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 3%
India 2 2%
Denmark 1 1%
Unknown 91 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 24%
Researcher 19 20%
Student > Master 17 18%
Student > Bachelor 10 10%
Student > Doctoral Student 8 8%
Other 13 13%
Unknown 7 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 39 40%
Biochemistry, Genetics and Molecular Biology 37 38%
Medicine and Dentistry 6 6%
Computer Science 1 1%
Nursing and Health Professions 1 1%
Other 5 5%
Unknown 8 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2017.
All research outputs
#1,443,389
of 12,367,469 outputs
Outputs from Genes & Development
#609
of 4,996 outputs
Outputs of similar age
#27,555
of 203,346 outputs
Outputs of similar age from Genes & Development
#10
of 54 outputs
Altmetric has tracked 12,367,469 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,996 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.7. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 203,346 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.