Chapter title |
Ancient DNA Extraction from Plants
|
---|---|
Chapter number | 10 |
Book title |
Ancient DNA
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-516-9_10 |
Pubmed ID | |
Book ISBNs |
978-1-61779-515-2, 978-1-61779-516-9
|
Authors |
Logan Kistler, Kistler, Logan |
Abstract |
A variety of protocols for DNA extraction from archaeological and paleobotanical plant specimens have been proposed. This is not surprising given the range of taxa and tissue types that may be preserved and the variety of conditions in which that preservation may take place. Commercially available DNA extraction kits can be used to recover ancient plant DNA, but modifications to standard approaches are often necessary to improve yield. In this chapter, I describe two protocols for extracting DNA from small amounts of ancient plant tissue. The CTAB protocol, which I recommend for use with single seeds, utilizes an incubation period in extraction buffer and subsequent chloroform extraction followed by DNA purification and suspension. The PTB protocol, which I recommend for use with gourd rind and similar tissues, utilizes an overnight incubation of pulverized tissue in extraction buffer, removal of the tissue by centrifugation, and DNA extraction from the buffer using commercial plant DNA extraction kits. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 1% |
United States | 1 | 1% |
Denmark | 1 | 1% |
Unknown | 85 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 22 | 25% |
Researcher | 22 | 25% |
Student > Bachelor | 12 | 14% |
Student > Postgraduate | 5 | 6% |
Student > Master | 4 | 5% |
Other | 9 | 10% |
Unknown | 14 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 42 | 48% |
Biochemistry, Genetics and Molecular Biology | 20 | 23% |
Arts and Humanities | 3 | 3% |
Environmental Science | 2 | 2% |
Business, Management and Accounting | 1 | 1% |
Other | 5 | 6% |
Unknown | 15 | 17% |