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Construction of sized eukaryotic cDNA libraries using low input of total environmental metatranscriptomic RNA

Overview of attention for article published in BMC Biotechnology, September 2014
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Mentioned by

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3 tweeters

Citations

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8 Dimensions

Readers on

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35 Mendeley
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Title
Construction of sized eukaryotic cDNA libraries using low input of total environmental metatranscriptomic RNA
Published in
BMC Biotechnology, September 2014
DOI 10.1186/1472-6750-14-80
Pubmed ID
Authors

Rajiv Kumar Yadav, Florian Barbi, Antoine Ziller, Patricia Luis, Roland Marmeisse, M Sudhakara Reddy, Laurence Fraissinet-Tachet

Abstract

Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by standard methods are represented by truncated cDNAs due to premature stoppage of reverse transcriptase activity and preferential cloning of short cDNAs.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 37%
Student > Ph. D. Student 6 17%
Student > Master 4 11%
Student > Doctoral Student 3 9%
Student > Postgraduate 3 9%
Other 3 9%
Unknown 3 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 43%
Biochemistry, Genetics and Molecular Biology 9 26%
Environmental Science 2 6%
Social Sciences 1 3%
Chemistry 1 3%
Other 1 3%
Unknown 6 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 September 2014.
All research outputs
#7,734,231
of 12,378,406 outputs
Outputs from BMC Biotechnology
#481
of 691 outputs
Outputs of similar age
#105,506
of 209,351 outputs
Outputs of similar age from BMC Biotechnology
#12
of 16 outputs
Altmetric has tracked 12,378,406 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 691 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 209,351 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.