Title |
Immobilization of carboxypeptidase from Sulfolobus solfataricuson magnetic nanoparticles improves enzyme stability and functionality in organic media
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Published in |
BMC Biotechnology, September 2014
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DOI | 10.1186/1472-6750-14-82 |
Pubmed ID | |
Authors |
Silvia Sommaruga, Elisabetta Galbiati, Jesus Peñaranda-Avila, Chiara Brambilla, Paolo Tortora, Miriam Colombo, Davide Prosperi |
Abstract |
Superparamagnetic iron oxide nanoparticles (MNP) offer several advantages for applications in biomedical and biotechnological research. In particular, MNP-based immobilization of enzymes allows high surface-to-volume ratio, good dispersibility, easy separation of enzymes from the reaction mixture, and reuse by applying an external magnetic field. In a biotechnological perspective, extremophilic enzymes hold great promise as they often can be used under non-conventional harsh conditions, which may result in substrate transformations that are not achievable with normal enzymes. This prompted us to investigate the effect of MNP bioconjugation on the catalytic properties of a thermostable carboxypeptidase from the hyperthermophilic archaeon Sulfolobus solfataricus (CPSso), which exhibits catalytic properties that are useful in synthetic processes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Sri Lanka | 1 | 2% |
Unknown | 43 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 23% |
Student > Master | 7 | 16% |
Student > Bachelor | 4 | 9% |
Professor > Associate Professor | 4 | 9% |
Researcher | 3 | 7% |
Other | 7 | 16% |
Unknown | 9 | 20% |
Readers by discipline | Count | As % |
---|---|---|
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Biochemistry, Genetics and Molecular Biology | 7 | 16% |
Chemistry | 5 | 11% |
Engineering | 3 | 7% |
Unspecified | 2 | 5% |
Other | 3 | 7% |
Unknown | 11 | 25% |