Title |
Smoking-induced chromosomal segregation anomalies identified by FISH analysis of sperm
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Published in |
Molecular Cytogenetics, September 2014
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DOI | 10.1186/s13039-014-0058-7 |
Pubmed ID | |
Authors |
Ciro Silveira Pereira, Maria Silvina Juchniuk de Vozzi, Silvio Avelino dos Santos, Maria Aparecida C Vasconcelos, Cláudia CP de Paz, Jeremy A Squire, Lucia Martelli |
Abstract |
Numerical chromosome aberrations in gametes are directly related to infertility and aneuploid embryos. Previous studies have shown that toxic substances from cigarette smoke induce structural and numerical chromosomal aberrations in vitro and could potentially increase levels of aneusomy in sperm. Moreover, increased levels of aneusomy in sperm are correlated with low implantation rates, spontaneous abortions and fetal losses. Studies of chromosome 3 in sperm suggest it may be more prone to segregation anomalies than other autosomes, but there has been no systematic investigation of the incidence of disomy for chromosome 3 in sperm derived from donor male smokers. The objective of this study was to use FISH to evaluate the influence of smoking on the levels of disomy for chromosomes X and Y, and to determine whether disomy levels for chromosome 3 were elevated in sperm derived from male smokers. |
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