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Engineering human ventricular heart muscles based on a highly efficient system for purification of human pluripotent stem cell-derived ventricular cardiomyocytes

Overview of attention for article published in Stem Cell Research & Therapy, September 2017
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1 tweeter
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Title
Engineering human ventricular heart muscles based on a highly efficient system for purification of human pluripotent stem cell-derived ventricular cardiomyocytes
Published in
Stem Cell Research & Therapy, September 2017
DOI 10.1186/s13287-017-0651-x
Pubmed ID
Authors

Bin Li, Hui Yang, Xiaochen Wang, Yongkun Zhan, Wei Sheng, Huanhuan Cai, Haoyang Xin, Qianqian Liang, Ping Zhou, Chao Lu, Ruizhe Qian, Sifeng Chen, Pengyuan Yang, Jianyi Zhang, Weinian Shou, Guoying Huang, Ping Liang, Ning Sun

Abstract

Most infarctions occur in the left anterior descending coronary artery and cause myocardium damage of the left ventricle. Although current pluripotent stem cells (PSCs) and directed cardiac differentiation techniques are able to generate fetal-like human cardiomyocytes, isolation of pure ventricular cardiomyocytes has been challenging. For repairing ventricular damage, we aimed to establish a highly efficient purification system to obtain homogeneous ventricular cardiomyocytes and prepare engineered human ventricular heart muscles in a dish. The purification system used TALEN-mediated genomic editing techniques to insert the neomycin or EGFP selection marker directly after the myosin light chain 2 (MYL2) locus in human pluripotent stem cells. Purified early ventricular cardiomyocytes were estimated by immunofluorescence, fluorescence-activated cell sorting, quantitative PCR, microelectrode array, and patch clamp. In subsequent experiments, the mixture of mature MYL2-positive ventricular cardiomyocytes and mesenchymal cells were cocultured with decellularized natural heart matrix. Histological and electrophysiology analyses of the formed tissues were performed 2 weeks later. Human ventricular cardiomyocytes were efficiently isolated based on the purification system using G418 or flow cytometry selection. When combined with the decellularized natural heart matrix as the scaffold, functional human ventricular heart muscles were prepared in a dish. These engineered human ventricular muscles can be great tools for regenerative therapy of human ventricular damage as well as drug screening and ventricular-specific disease modeling in the future.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 22%
Student > Ph. D. Student 6 19%
Researcher 4 13%
Professor 3 9%
Student > Postgraduate 2 6%
Other 7 22%
Unknown 3 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 38%
Agricultural and Biological Sciences 4 13%
Medicine and Dentistry 4 13%
Earth and Planetary Sciences 1 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 4 13%
Unknown 6 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2017.
All research outputs
#9,711,545
of 12,657,345 outputs
Outputs from Stem Cell Research & Therapy
#741
of 1,075 outputs
Outputs of similar age
#182,580
of 271,591 outputs
Outputs of similar age from Stem Cell Research & Therapy
#35
of 57 outputs
Altmetric has tracked 12,657,345 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,075 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,591 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.