Title |
CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome
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Published in |
Scientific Reports, October 2017
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DOI | 10.1038/s41598-017-11976-6 |
Pubmed ID | |
Authors |
José Luis Torán, Susana Aguilar, Juan Antonio López, Carlos Torroja, Juan Antonio Quintana, Cesar Santiago, José Luis Abad, Patricia Gomes-Alves, Andrés Gonzalez, Juan Antonio Bernal, Luis Jesús Jiménez-Borreguero, Paula Marques Alves, Luis R-Borlado, Jesús Vázquez, Antonio Bernad |
Abstract |
Studies in recent years have established that the principal effects in cardiac cell therapy are associated with paracrine/autocrine factors. We combined several complementary techniques to define human cardiac progenitor cell (CPC) secretome constituted by 914 proteins/genes; 51% of these are associated with the exosomal compartment. To define the set of proteins specifically or highly differentially secreted by CPC, we compared human mesenchymal stem cells and dermal fibroblasts; the study defined a group of growth factors, cytokines and chemokines expressed at high to medium levels by CPC. Among them, IL-1, GROa (CXCL1), CXCL6 (GCP2) and IL-8 are examples whose expression was confirmed by most techniques used. ELISA showed that CXCL6 is significantly overexpressed in CPC conditioned medium (CM) (18- to 26-fold) and western blot confirmed expression of its receptors CXCR1 and CXCR2. Addition of anti-CXCL6 completely abolished migration in CPC-CM compared with anti-CXCR2, which promoted partial inhibition, and anti-CXCR1, which was inefficient. Anti-CXCL6 also significantly inhibited CPC CM angiogenic activity. In vivo evaluation also supported a relevant role for angiogenesis. Altogether, these results suggest a notable angiogenic potential in CPC-CM and identify CXCL6 as an important paracrine factor for CPC that signals mainly through CXCR2. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 53 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 19% |
Student > Ph. D. Student | 8 | 15% |
Student > Master | 5 | 9% |
Student > Doctoral Student | 4 | 8% |
Student > Bachelor | 2 | 4% |
Other | 8 | 15% |
Unknown | 16 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 12 | 23% |
Agricultural and Biological Sciences | 4 | 8% |
Medicine and Dentistry | 4 | 8% |
Nursing and Health Professions | 3 | 6% |
Materials Science | 2 | 4% |
Other | 9 | 17% |
Unknown | 19 | 36% |