Title |
A novel early precursor cell population from rat bone marrow promotes angiogenesis in vitro
|
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Published in |
BMC Molecular and Cell Biology, March 2014
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DOI | 10.1186/1471-2121-15-12 |
Pubmed ID | |
Authors |
Andreas Brandl, Quan Yuan, Anja M Boos, Justus P Beier, Andreas Arkudas, Ulrich Kneser, Raymund E Horch, Oliver Bleiziffer |
Abstract |
Some studies demonstrated therapeutic angiogenesis attributable to the effects of endothelial progenitor cells (EPC), others have reported disappointing results. This may be due to the fact that EPC populations used in these contradictory studies were selected and defined by highly variable and differing experimental protocols. Indeed, the isolation and reliable characterization of ex vivo differentiated EPC raises considerable problems due to the fact there is no biomarker currently available to specifically identify EPC exclusively. On the other hand traditional differentiation of primary immature bone marrow cells towards the endothelial lineage is a time-consuming process of up to 5 weeks. To circumvent these shortcomings, we herein describe a facile method to isolate and enrich a primary cell population from rat bone marrow, combining differential attachment methodology with cell sorting technology. |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
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Demographic breakdown
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Researcher | 3 | 20% |
Student > Doctoral Student | 2 | 13% |
Other | 2 | 13% |
Professor > Associate Professor | 2 | 13% |
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Other | 4 | 27% |
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Veterinary Science and Veterinary Medicine | 1 | 7% |
Other | 2 | 13% |
Unknown | 1 | 7% |