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Protein Chromatography

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Cover of 'Protein Chromatography'

Table of Contents

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    Book Overview
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    Chapter 1 A Digest of Protein Purification
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    Chapter 2 Gel-Filtration Chromatography
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    Chapter 3 Immunoaffinity Chromatography
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    Chapter 4 Avoiding Proteolysis During Protein Chromatography
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    Chapter 5 Scale-Up of Protein Purification: Downstream Processing Issues
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    Chapter 6 Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources
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    Chapter 7 Engineering Protein Stability
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    Chapter 8 Microfluidics in Protein Chromatography
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    Chapter 9 Tagging recombinant proteins to enhance solubility and aid purification.
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    Chapter 10 Storage and Lyophilisation of Pure Proteins
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    Chapter 11 Differential Precipitation and Solubilization of Proteins
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    Chapter 12 Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase
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    Chapter 13 Protein Quantitation and Analysis of Purity
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    Chapter 14 Purification of proteins fused to glutathione S-transferase.
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    Chapter 15 Purification of Proteins Fused to Maltose-Binding Protein
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    Chapter 16 Purification of Proteins from Baculovirus-Infected Insect Cells
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    Chapter 17 Purification of Poly-Histidine-Tagged Proteins
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    Chapter 18 Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry
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    Chapter 19 Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins
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    Chapter 20 Purification of Antibodies Using Affinity Chromatography
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    Chapter 21 Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multiantigen Immunized Chickens
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    Chapter 22 Measuring Protein–Protein Interactions Using Biacore
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    Chapter 23 Ultra-Performance Liquid Chromatography–Mass Spectrometry of Proteins
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    Chapter 24 Hydrophobic Interaction Chromatography
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    Chapter 25 Fast protein liquid chromatography.
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    Chapter 26 Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS
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    Chapter 27 Shotgun Proteomics: A Relative Quantitative Approach Using Off-Gel Electrophoresis and LC-MS/MS
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    Chapter 28 Clinical Proteomics: Liquid Chromatography–Mass Spectrometry Purification Systems
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    Chapter 29 Strategies for the purification of membrane proteins.
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    Chapter 30 A Multi-Step Chromatographic Strategy to Purify Three Fungal Endo-β-Glucanases
Attention for Chapter 4: Avoiding Proteolysis During Protein Chromatography
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Citations

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Chapter title
Avoiding Proteolysis During Protein Chromatography
Chapter number 4
Book title
Protein Chromatography
Published by
Humana Press, January 2011
DOI 10.1007/978-1-60761-913-0_4
Book ISBNs
978-1-60761-912-3, 978-1-60761-913-0
Authors

Barry J. Ryan

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 34 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 17%
Student > Ph. D. Student 5 14%
Professor 4 11%
Student > Bachelor 3 9%
Student > Doctoral Student 2 6%
Other 9 26%
Unknown 6 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 34%
Biochemistry, Genetics and Molecular Biology 10 29%
Medicine and Dentistry 3 9%
Economics, Econometrics and Finance 1 3%
Environmental Science 1 3%
Other 2 6%
Unknown 6 17%