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Imaging Flow Cytometry

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Cover of 'Imaging Flow Cytometry'

Table of Contents

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    Book Overview
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    Chapter 1 Quantitative Functional Morphology by Imaging Flow Cytometry
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    Chapter 2 Principles of Amnis Imaging Flow Cytometry
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    Chapter 3 Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch
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    Chapter 4 Applications of Imaging Flow Cytometry for Microalgae
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    Chapter 5 The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry
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    Chapter 6 Quantitation of Chromosome Damage by Imaging Flow Cytometry
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    Chapter 7 Fluorescent In Situ Hybridization in Suspension by Imaging Flow Cytometry
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    Chapter 8 Analysis of Nucleocytoplasmic Protein Shuttling by Imaging Flow Cytometry
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    Chapter 9 Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation
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    Chapter 10 Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy
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    Chapter 11 Studying T Cells N-Glycosylation by Imaging Flow Cytometry
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    Chapter 12 Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry
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    Chapter 13 Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity
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    Chapter 14 Imaging Flow Cytometry
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    Chapter 15 Accurate Assessment of Cell Death by Imaging Flow Cytometry
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    Chapter 16 Imaging Flow Cytometry
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    Chapter 17 FlowCam: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry
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    Chapter 18 Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry
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    Chapter 19 Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes
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    Chapter 20 Sickle Cell Imaging Flow Cytometry Assay (SIFCA)
Attention for Chapter 2: Principles of Amnis Imaging Flow Cytometry
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Chapter title
Principles of Amnis Imaging Flow Cytometry
Chapter number 2
Book title
Imaging Flow Cytometry
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3302-0_2
Pubmed ID
Book ISBNs
978-1-4939-3300-6, 978-1-4939-3302-0
Authors

David A. Basiji

Abstract

Over the last decade imaging flow cytometry (IFC) has become an established technique, as evidenced by its use in over 500 peer-reviewed scientific articles. Nevertheless, it is still an emerging technique with an installed base of less than 5 % that of conventional flow cytometers. In parallel with its adoption, the technology has evolved rapidly, increasing in speed, sensitivity, and real-time data analysis capacity by over an order of magnitude since its introduction. This chapter summarizes IFC's basic principles of operation and describes the current state of the art.

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Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Student > Bachelor 4 19%
Other 1 5%
Professor 1 5%
Student > Master 1 5%
Other 2 10%
Unknown 7 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 19%
Agricultural and Biological Sciences 3 14%
Engineering 2 10%
Immunology and Microbiology 2 10%
Environmental Science 1 5%
Other 2 10%
Unknown 7 33%