| Chapter title |
Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation
|
|---|---|
| Chapter number | 9 |
| Book title |
Imaging Flow Cytometry
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3302-0_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3300-6, 978-1-4939-3302-0
|
| Authors |
Adam S. Venable, Andrea L. Henning, Eric A. Prado, Brian K. McFarlin |
| Abstract |
Existing methods of assessing monocyte inflammatory cytokine (IL-1β, IL-6, IL-8, and TNF-α) response to in vitro lipopolysaccharide (LPS) stimulation lack the ability to simultaneously detect intracellular mRNA and protein. This procedure takes advantage of new methodologies and instrumentation to simultaneously measure intracellular TNF-α mRNA and protein in CD14(+) monocytes after 1, 3, and 6 h of LPS stimulation. By assessing multiple timepoints, we are able to discern how LPS stimulation affects the temporal relationship between TNF-α mRNA and protein. By using image-based flow cytometry it is possible to co-localize mRNA and protein signals to identify the length of incubation that is needed to initiate protein translation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 38% |
| Professor > Associate Professor | 1 | 13% |
| Other | 1 | 13% |
| Student > Master | 1 | 13% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 25% |
| Immunology and Microbiology | 2 | 25% |
| Sports and Recreations | 1 | 13% |
| Agricultural and Biological Sciences | 1 | 13% |
| Unknown | 2 | 25% |