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A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis

Overview of attention for article published in Revista do Instituto de Medicina Tropical de São Paulo, September 2017
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Title
A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis
Published in
Revista do Instituto de Medicina Tropical de São Paulo, September 2017
DOI 10.1590/s1678-9946201759066
Pubmed ID
Authors

Saeedeh Shamsaddini, Mohammad Ali Mohammadi, Seyed Reza Mirbadie, Sima Rostami, Mansoureh Dehghani, Balal Sadeghi, Majid Fasihi Harandi

Abstract

Canine taeniids are among the major tapeworms with remarkable medical and economic significance. Reliable diagnosis and differentiation of dog taeniids using simple and sensitive tools are of paramount importance for establishing an efficient surveillance system. Microsatellites as abundant unique tandem repeats of short DNA motifs are useful genetic markers for molecular epidemiological studies. The purpose of the present study was to find a primer pair for rapid differentiation of major tapeworms of dogs, Taenia hydatigena, T. multiceps, T. ovis and Echinococcus granulosus, by screening existing nucleotide data. All the mitochondrial genome records as well as non-coding ITS1 sequences of Taeniidae species were downloaded from Nucleotide database from NCBI. For prediction and analysis of potential loci of STR/SSR in ITS1 as well as mitochondrial regions, we used ChloroMitoSSRDB 2.0 and GMATo v1.2. software. Different tapeworm species were categorized according to different motif sequences and type and size of each microsatellite locus. Three primer sets were designed and tested for differentiating taeniid species and evaluated in a conventional PCR system. Four taeniid species were successfully differentiated using a primer pair in a simple conventional PCR system. We predicted 2-19 and 1-4 microsatellite loci in ITS1 and mitochondrial genome, respectively. In ITS1, 41 Di and 21 Tri motifs were found in the taeniids while the majority of the motifs in the mitochondrial genome were Tetra (89) and Tri (70). It is documented that the number and diversity of microsatellite loci is higher in nuclear ITS1 region than mostly coding mitochondrial genome.

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Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 30%
Student > Doctoral Student 2 10%
Other 2 10%
Student > Ph. D. Student 2 10%
Professor 2 10%
Other 4 20%
Unknown 2 10%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 5 25%
Medicine and Dentistry 3 15%
Biochemistry, Genetics and Molecular Biology 2 10%
Agricultural and Biological Sciences 2 10%
Immunology and Microbiology 1 5%
Other 3 15%
Unknown 4 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2017.
All research outputs
#10,527,346
of 11,880,222 outputs
Outputs from Revista do Instituto de Medicina Tropical de São Paulo
#212
of 245 outputs
Outputs of similar age
#230,537
of 273,533 outputs
Outputs of similar age from Revista do Instituto de Medicina Tropical de São Paulo
#4
of 7 outputs
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So far Altmetric has tracked 245 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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