Chapter title |
Protein Arrays I: Antibody Arrays
|
---|---|
Chapter number | 19 |
Book title |
Functional Genomics
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7231-9_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7230-2, 978-1-4939-7231-9
|
Authors |
Yulin Yuan, Zuan-Tao Lin, Hongting Wang, Xia Hong, Mikala Heon, Tianfu Wu |
Abstract |
Antibody arrays represent one of the very early protein array systems where antibodies are used to capture and detect target proteins in a high-throughput platform. The development of high-quality antibodies, nanomaterial-based novel detection probes, as well as innovative imaging technologies and computational tools has tremendously improved the sensitivity, specificity, and robustness of antibody arrays during the past decade. In this protocol we will incorporate the most updated innovations and developments of antibody arrays into the step-by-step experimental procedures. This includes antibody printing, sample preparation, array detection, as well as imaging and data analysis. Antibody array could be used for cytokine profiling or mapping of phosphorylation, glycosylation, or other post-translational modifications of target proteins. |
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