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| Chapter title |
Quantification of NMDAR Subunit Genes Expression by qRT-PCR
|
|---|---|
| Chapter number | 2 |
| Book title |
NMDA Receptors
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7321-7_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7320-0, 978-1-4939-7321-7
|
| Authors |
Emilie Pallesi-Pocachard |
| Abstract |
Transcription is the initial and generally the most sensitive step to cellular needs and environmental cues. Thus, it serves as a major mechanism controlling gene expression. Using reverse-transcription quantitative polymerase chain reaction technology (RT-qPCR), we will present how to quantify the transcriptional expression of NMDARs subunits during brain development and in both healthy and pathological conditions. |