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Plant Fungal Pathogens

Overview of attention for book
Cover of 'Plant Fungal Pathogens'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Fungal ABC Transporter Deletion and Localization Analysis
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    Chapter 2 Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens- Mediated Transformation
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    Chapter 3 Activity-Based Protein Profiling of Infected Plants
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    Chapter 4 The Use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves
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    Chapter 5 Plant Fungal Pathogens
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    Chapter 6 Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway ® -Compatible Binary Potato Virus X-Expression Vector
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    Chapter 7 Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens
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    Chapter 8 Real-Time PCR for the Quantification of Fungi In Planta
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    Chapter 9 Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
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    Chapter 10 Atomic Force Microscopy: A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates
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    Chapter 11 Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors
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    Chapter 12 Characterization of Plant-Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens
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    Chapter 13 Heterologous Production of Fungal Effectors in Pichia pastoris
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    Chapter 14 The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta
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    Chapter 15 Plant Fungal Pathogens
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    Chapter 16 Targeted Gene Replacement in Fungi Using a Split-Marker Approach
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    Chapter 17 Massively parallel sequencing technology in pathogenic microbes.
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    Chapter 18 Confocal Microscopy in Plant–Pathogen Interactions
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    Chapter 19 The Use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data
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    Chapter 20 Population biology of fungal plant pathogens.
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    Chapter 21 Polyethylene Glycol (PEG)-Mediated Transformation in Filamentous Fungal Pathogens
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    Chapter 22 In Vitro Induction of Infection-Related Hyphal Structures in Plant Pathogenic Fungi
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    Chapter 23 Fungicide Resistance Assays for Fungal Plant Pathogens
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    Chapter 24 Identification of lipid-binding effectors.
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    Chapter 25 In Silico Identification and Characterization of Effector Catalogs
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    Chapter 26 Horizontal Transfer of Supernumerary Chromosomes in Fungi
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    Chapter 27 The Induction of Mycotoxins by Trichothecene Producing Fusarium Species
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    Chapter 28 Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics
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    Chapter 29 Assessment of Autophagosome Formation by Transmission Electron Microscopy
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    Chapter 30 Fungal Plant Pathogen Detection in Plant and Soil Samples Using DNA Macroarrays
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    Chapter 31 Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors
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    Chapter 32 A yeast secretion trap assay for identification of secreted proteins from eukaryotic phytopathogens and their plant hosts.
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    Chapter 33 Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes
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    Chapter 34 Multigene phylogenetic analyses to delimit new species in fungal plant pathogens.
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    Chapter 35 MAP Kinase Phosphorylation and cAMP Assessment in Fungi
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    Chapter 36 A One-Step Affinity-Purification Protocol to Purify NB-LRR Immune Receptors from Plants That Mediate Resistance to Fungal Pathogens
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    Chapter 37 Karyotyping Methods for Fungi
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    Chapter 38 Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique
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    Chapter 39 Gene Cloning Using Degenerate Primers and Genome Walking
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    Chapter 40 Construction of Hairpin RNA-Expressing Vectors for RNA-Mediated Gene Silencing in Fungi
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    Chapter 41 An Unbiased Method for the Quantitation of Disease Phenotypes Using a Custom-Built Macro Plugin for the Program ImageJ
Attention for Chapter 31: Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors
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Chapter title
Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors
Chapter number 31
Book title
Plant Fungal Pathogens
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-501-5_31
Pubmed ID
Book ISBNs
978-1-61779-500-8, 978-1-61779-501-5
Authors

Parthasarathy Santhanam, Santhanam P

Abstract

Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 35%
Student > Master 5 25%
Student > Ph. D. Student 4 20%
Other 1 5%
Student > Bachelor 1 5%
Other 0 0%
Unknown 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 65%
Biochemistry, Genetics and Molecular Biology 2 10%
Immunology and Microbiology 2 10%
Nursing and Health Professions 1 5%
Unknown 2 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 May 2014.
All research outputs
#17,682,134
of 22,701,287 outputs
Outputs from Methods in molecular biology
#7,160
of 13,076 outputs
Outputs of similar age
#191,367
of 244,143 outputs
Outputs of similar age from Methods in molecular biology
#313
of 474 outputs
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So far Altmetric has tracked 13,076 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
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We're also able to compare this research output to 474 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.