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Difference Gel Electrophoresis

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Cover of 'Difference Gel Electrophoresis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Two-Dimensional Gel Electrophoresis and 2D-DIGE
  3. Altmetric Badge
    Chapter 2 Comparative DIGE Proteomics
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    Chapter 3 2D-DIGE and Fluorescence Image Analysis
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    Chapter 4 DIGE Analysis Software and Protein Identification Approaches
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    Chapter 5 Native DIGE: Efficient Tool to Elucidate Protein Interactomes
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    Chapter 6 Comparative Two-Dimensional Fluorescence Gel Electrophoresis
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    Chapter 7 DIGE-Based Phosphoproteomic Analysis
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    Chapter 8 DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
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    Chapter 9 Comparative 3-Sample DIGE Analysis of Skeletal Muscles
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    Chapter 10 DIGE Analysis of ProteoMinerTM Fractionated Serum/Plasma Samples
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    Chapter 11 DIGE Analysis of Human Tissues
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    Chapter 12 DIGE Analysis of Animal Tissues
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    Chapter 13 Rapid 2D DIGE Proteomic Analysis of Mouse Liver
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    Chapter 14 Proteomic Analysis of Lung Tissue by DIGE
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    Chapter 15 Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis
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    Chapter 16 DIGE Analysis of Fish Tissues
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    Chapter 17 Protein Digestion for DIGE Analysis
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    Chapter 18 Subcellular Fractionation for DIGE-Based Proteomics
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    Chapter 19 DIGE Analysis of Immunodepleted Plasma
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    Chapter 20 Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments Using Enzyme Assays
  22. Altmetric Badge
    Chapter 21 Enzyme Assay Methods to Validate DIGE Proteomics Data
  23. Altmetric Badge
    Chapter 22 Immunoblot Analysis of DIGE-Based Proteomics
  24. Altmetric Badge
    Chapter 23 Immunofluorescence Microscopy for DIGE-Based Proteomics
Attention for Chapter 15: Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis
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Chapter title
Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis
Chapter number 15
Book title
Difference Gel Electrophoresis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7268-5_15
Pubmed ID
Book ISBNs
978-1-4939-7267-8, 978-1-4939-7268-5
Authors

Ashling Holland

Abstract

Comparative tissue proteomics aims to analyze alterations of the proteome in response to a stimulus. Two-dimensional difference gel electrophoresis (2D-DIGE) is a modified and advanced form of 2D gel electrophoresis. DIGE is a powerful biochemical method that compares two or three protein samples on the same analytical gel, and can be used to establish differentially expressed protein levels between healthy normal and diseased pathological tissue sample groups. Minimal DIGE labeling can be used via a 2-dye system with Cy3 and Cy5 or a 3-dye system with Cy2, Cy3, and Cy5 to fluorescently label samples with CyDye flours pre-electrophoresis. DIGE circumvents gel-to-gel variability by multiplexing samples to a single gel and through the use of a pooled internal standard for normalization. This form of quantitative high-resolution proteomics facilitates the comparative analysis and evaluation of tissue protein compositions. Comparing tissue groups under different conditions is crucially important for advancing the biomedical field by characterization of cellular processes, understanding pathophysiological development and tissue biomarker discovery. This chapter discusses 2D-DIGE as a comparative tissue proteomic technique and describes in detail the experimental steps required for comparative proteomic analysis employing both options of 2-dye and 3-dye DIGE minimal labeling.

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Mendeley readers

Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 33%
Researcher 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Unknown 2 67%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
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#18,573,839
of 23,005,189 outputs
Outputs from Methods in molecular biology
#7,961
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Outputs of similar age
#330,439
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#950
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