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Mendeley readers
| Chapter title |
DIGE-Based Phosphoproteomic Analysis
|
|---|---|
| Chapter number | 7 |
| Book title |
Difference Gel Electrophoresis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7268-5_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7267-8, 978-1-4939-7268-5
|
| Authors |
Taras Stasyk, Lukas Alfons Huber |
| Abstract |
Here, we describe the detailed step-by-step protocol for detection of phosphoproteins in two-dimensional difference gel electrophoresis (DIGE) gels. A standard DIGE protocol is combined with subsequent post-staining with phosphospecific fluorescent dye. The combination of these two methods complements DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitive and accurate quantification of the differentially regulated phosphoproteins in biological samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 50% |
| Researcher | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |
| Agricultural and Biological Sciences | 1 | 50% |