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Difference Gel Electrophoresis

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Cover of 'Difference Gel Electrophoresis'

Table of Contents

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    Book Overview
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    Chapter 1 Two-Dimensional Gel Electrophoresis and 2D-DIGE
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    Chapter 2 Comparative DIGE Proteomics
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    Chapter 3 2D-DIGE and Fluorescence Image Analysis
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    Chapter 4 DIGE Analysis Software and Protein Identification Approaches
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    Chapter 5 Native DIGE: Efficient Tool to Elucidate Protein Interactomes
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    Chapter 6 Comparative Two-Dimensional Fluorescence Gel Electrophoresis
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    Chapter 7 DIGE-Based Phosphoproteomic Analysis
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    Chapter 8 DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
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    Chapter 9 Comparative 3-Sample DIGE Analysis of Skeletal Muscles
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    Chapter 10 DIGE Analysis of ProteoMinerTM Fractionated Serum/Plasma Samples
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    Chapter 11 DIGE Analysis of Human Tissues
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    Chapter 12 DIGE Analysis of Animal Tissues
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    Chapter 13 Rapid 2D DIGE Proteomic Analysis of Mouse Liver
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    Chapter 14 Proteomic Analysis of Lung Tissue by DIGE
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    Chapter 15 Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis
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    Chapter 16 DIGE Analysis of Fish Tissues
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    Chapter 17 Protein Digestion for DIGE Analysis
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    Chapter 18 Subcellular Fractionation for DIGE-Based Proteomics
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    Chapter 19 DIGE Analysis of Immunodepleted Plasma
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    Chapter 20 Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments Using Enzyme Assays
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    Chapter 21 Enzyme Assay Methods to Validate DIGE Proteomics Data
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    Chapter 22 Immunoblot Analysis of DIGE-Based Proteomics
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    Chapter 23 Immunofluorescence Microscopy for DIGE-Based Proteomics
Attention for Chapter 3: 2D-DIGE and Fluorescence Image Analysis
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Chapter title
2D-DIGE and Fluorescence Image Analysis
Chapter number 3
Book title
Difference Gel Electrophoresis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7268-5_3
Pubmed ID
Book ISBNs
978-1-4939-7267-8, 978-1-4939-7268-5
Authors

Elisa Robotti, Emilio Marengo

Abstract

2D-DIGE is still a very widespread technique in proteomics for the identification of panels of biomarkers, allowing to tackle with some important drawback of classical two-dimensional gel-electrophoresis. However, once 2D-gels are obtained, they must undergo a quite articulated multistep image analysis procedure before the final differential analysis via statistical mono- and multivariate methods. Here, the main steps of image analysis software are described and the most recent procedures reported in the literature are briefly presented.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 1 14%
Student > Bachelor 1 14%
Professor 1 14%
Student > Ph. D. Student 1 14%
Researcher 1 14%
Other 0 0%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 1 14%
Unknown 4 57%