↓ Skip to main content

Difference Gel Electrophoresis

Overview of attention for book
Cover of 'Difference Gel Electrophoresis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Two-Dimensional Gel Electrophoresis and 2D-DIGE
  3. Altmetric Badge
    Chapter 2 Comparative DIGE Proteomics
  4. Altmetric Badge
    Chapter 3 2D-DIGE and Fluorescence Image Analysis
  5. Altmetric Badge
    Chapter 4 DIGE Analysis Software and Protein Identification Approaches
  6. Altmetric Badge
    Chapter 5 Native DIGE: Efficient Tool to Elucidate Protein Interactomes
  7. Altmetric Badge
    Chapter 6 Comparative Two-Dimensional Fluorescence Gel Electrophoresis
  8. Altmetric Badge
    Chapter 7 DIGE-Based Phosphoproteomic Analysis
  9. Altmetric Badge
    Chapter 8 DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
  10. Altmetric Badge
    Chapter 9 Comparative 3-Sample DIGE Analysis of Skeletal Muscles
  11. Altmetric Badge
    Chapter 10 DIGE Analysis of ProteoMinerTM Fractionated Serum/Plasma Samples
  12. Altmetric Badge
    Chapter 11 DIGE Analysis of Human Tissues
  13. Altmetric Badge
    Chapter 12 DIGE Analysis of Animal Tissues
  14. Altmetric Badge
    Chapter 13 Rapid 2D DIGE Proteomic Analysis of Mouse Liver
  15. Altmetric Badge
    Chapter 14 Proteomic Analysis of Lung Tissue by DIGE
  16. Altmetric Badge
    Chapter 15 Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis
  17. Altmetric Badge
    Chapter 16 DIGE Analysis of Fish Tissues
  18. Altmetric Badge
    Chapter 17 Protein Digestion for DIGE Analysis
  19. Altmetric Badge
    Chapter 18 Subcellular Fractionation for DIGE-Based Proteomics
  20. Altmetric Badge
    Chapter 19 DIGE Analysis of Immunodepleted Plasma
  21. Altmetric Badge
    Chapter 20 Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments Using Enzyme Assays
  22. Altmetric Badge
    Chapter 21 Enzyme Assay Methods to Validate DIGE Proteomics Data
  23. Altmetric Badge
    Chapter 22 Immunoblot Analysis of DIGE-Based Proteomics
  24. Altmetric Badge
    Chapter 23 Immunofluorescence Microscopy for DIGE-Based Proteomics
Attention for Chapter 2: Comparative DIGE Proteomics
Altmetric Badge

Citations

dimensions_citation
5 Dimensions

Readers on

mendeley
39 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Comparative DIGE Proteomics
Chapter number 2
Book title
Difference Gel Electrophoresis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7268-5_2
Pubmed ID
Book ISBNs
978-1-4939-7267-8, 978-1-4939-7268-5
Authors

Kay Ohlendieck

Abstract

Gel-based proteomics has been widely used for the systematic cataloging of the protein constituents of defined biofluids, purified organelles, individual cell types, heterogeneous tissues and isolated organs, as well as being applied to comparative biochemical and biomedical analyses of complex biological specimens. Of the many electrophoretic techniques used in modern biochemical approaches, large-scale protein separation by difference gel electrophoresis (DIGE) has established itself as the most powerful analytical tool in comparative proteomics. Both 2-dye and 3-dye fluorescence systems with minimal or saturation labeling are routinely used. This chapter briefly describes the technical advantages of the pre-electrophoretic fluorescent labeling technique and discusses the bioanalytical usefulness of this highly successful electrophoretic method.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 21%
Student > Bachelor 5 13%
Student > Ph. D. Student 4 10%
Student > Master 4 10%
Professor > Associate Professor 2 5%
Other 5 13%
Unknown 11 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 21%
Biochemistry, Genetics and Molecular Biology 6 15%
Medicine and Dentistry 5 13%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Arts and Humanities 1 3%
Other 3 8%
Unknown 14 36%