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Mendeley readers
| Chapter title |
Rapid 2D DIGE Proteomic Analysis of Mouse Liver
|
|---|---|
| Chapter number | 13 |
| Book title |
Difference Gel Electrophoresis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7268-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7267-8, 978-1-4939-7268-5
|
| Authors |
Shotaro Kamata, Isao Ishii |
| Abstract |
Several years have passed since LC-MS(/MS) became the mainstream for proteomic analysis; however, conventional 2D DIGE (two-dimensional difference gel electrophoresis) continues to be an important technology that enables rapid and direct visualization of hundreds to thousands of proteins and their quantitative analyses. We can get global proteomic views using 2D DIGE within 3 days, and then identify proteins with differential expression levels using MALDI-TOF/MS and MASCOT search engine within a week. Here, we describe our routine 2D DIGE proteomic analysis of the liver isolated from mice in pathological conditions. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 50% |
| Lecturer | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 1 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |