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X Demographics
Mendeley readers
Attention Score in Context
| Title |
3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy
|
|---|---|
| Published in |
Nature Protocols, April 2014
|
| DOI | 10.1038/nprot.2014.087 |
| Pubmed ID | |
| Authors |
Liang Gao, Lin Shao, Bi-Chang Chen, Eric Betzig |
| Abstract |
3D live imaging is important for a better understanding of biological processes, but it is challenging with current techniques such as spinning-disk confocal microscopy. Bessel beam plane illumination microscopy allows high-speed 3D live fluorescence imaging of living cellular and multicellular specimens with nearly isotropic spatial resolution, low photobleaching and low photodamage. Unlike conventional fluorescence imaging techniques that usually have a unique operation mode, Bessel plane illumination has several modes that offer different performance with different imaging metrics. To achieve optimal results from this technique, the appropriate operation mode needs to be selected and the experimental setting must be optimized for the specific application and associated sample properties. Here we explain the fundamental working principles of this technique, discuss the pros and cons of each operational mode and show through examples how to optimize experimental parameters. We also describe the procedures needed to construct, align and operate a Bessel beam plane illumination microscope by using our previously reported system as an example, and we list the necessary equipment to build such a microscope. Assuming all components are readily available, it would take a person skilled in optical instrumentation ∼1 month to assemble and operate a microscope according to this protocol. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 25% |
| Germany | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 515 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 9 | 2% |
| United Kingdom | 7 | 1% |
| Germany | 5 | <1% |
| Korea, Republic of | 2 | <1% |
| Italy | 2 | <1% |
| France | 2 | <1% |
| Canada | 2 | <1% |
| Spain | 2 | <1% |
| Israel | 1 | <1% |
| Other | 5 | <1% |
| Unknown | 478 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 142 | 28% |
| Researcher | 112 | 22% |
| Student > Master | 40 | 8% |
| Student > Bachelor | 40 | 8% |
| Professor | 27 | 5% |
| Other | 86 | 17% |
| Unknown | 68 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 128 | 25% |
| Engineering | 98 | 19% |
| Agricultural and Biological Sciences | 92 | 18% |
| Biochemistry, Genetics and Molecular Biology | 55 | 11% |
| Chemistry | 18 | 3% |
| Other | 49 | 10% |
| Unknown | 75 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 35. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2021.
All research outputs
#1,157,199
of 25,635,728 outputs
Outputs from Nature Protocols
#307
of 2,959 outputs
Outputs of similar age
#11,123
of 241,951 outputs
Outputs of similar age from Nature Protocols
#5
of 48 outputs
Altmetric has tracked 25,635,728 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,959 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 16.6. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 241,951 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.