↓ Skip to main content

Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, September 2014
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
15 Dimensions

Readers on

mendeley
98 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
Published in
Memórias do Instituto Oswaldo Cruz, September 2014
DOI 10.1590/0074-0276140046
Pubmed ID
Authors

Ronaldo Bragança Martins Júnior, Sharon Carney, Daniel Goldemberg, Lucas Bonine, Liliana Cruz Spano, Marilda Siqueira, Rita Elizabeth Checon

Abstract

Viruses are the major contributors to the morbidity and mortality of upper and lower acute respiratory infections (ARIs) for all age groups. The aim of this study was to determine the frequencies for a large range of respiratory viruses using a sensitive molecular detection technique in specimens from outpatients of all ages with ARIs. Nasopharyngeal aspirates were obtained from 162 individuals between August 2007-August 2009. Twenty-three pathogenic respiratory agents, 18 respiratory viruses and five bacteria were investigated using multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence assay (IIF). Through IIF, 33 (20.4%) specimens with respiratory virus were recognised, with influenza virus representing over half of the positive samples. Through a multiplex real-time RT-PCR assay, 88 (54.3%) positive samples were detected; the most prevalent respiratory viral pathogens were influenza, human rhinovirus and respiratory syncytial virus (RSV). Six cases of viral co-detection were observed, mainly involving RSV. The use of multiplex real-time RT-PCR increased the viral detection by 33.9% and revealed a larger number of respiratory viruses implicated in ARI cases, including the most recently described respiratory viruses [human bocavirus, human metapneumovirus, influenza A (H1N1) pdm09 virus, human coronavirus (HCoV) NL63 and HCoV HKU1].

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 1%
Unknown 97 99%

Demographic breakdown

Readers by professional status Count As %
Student > Master 22 22%
Student > Bachelor 17 17%
Researcher 15 15%
Student > Ph. D. Student 10 10%
Student > Doctoral Student 7 7%
Other 15 15%
Unknown 12 12%
Readers by discipline Count As %
Medicine and Dentistry 27 28%
Biochemistry, Genetics and Molecular Biology 16 16%
Agricultural and Biological Sciences 13 13%
Immunology and Microbiology 8 8%
Nursing and Health Professions 4 4%
Other 12 12%
Unknown 18 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2020.
All research outputs
#11,949,854
of 18,838,632 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#774
of 1,236 outputs
Outputs of similar age
#115,466
of 221,832 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#7
of 17 outputs
Altmetric has tracked 18,838,632 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 221,832 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.