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dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridization

Overview of attention for article published in BMC Genomics, October 2017
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (69th percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

Mentioned by

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3 X users
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1 patent

Citations

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4 Dimensions

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22 Mendeley
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Title
dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridization
Published in
BMC Genomics, October 2017
DOI 10.1186/s12864-017-4159-7
Pubmed ID
Authors

Yanfeng Zhang, Jun Song, Kenneth Day, Devin Absher

Abstract

Targeted sequencing is a powerful tool with broad application in both basic and translational sciences. Relatively low on-target rates for most current targeted sequencing studies influence the required coverage and data quality for subsequent applications. We present an improved targeted sequencing method that uses two rounds of in solution hybridization with probes synthesized from genomic clone templates, termed dCATCH-Seq. Independent captures of two large continuous genomic regions across three cell types within the human major histocompatibility complex (MHC) that spans ~3.5 Mb and a ~250 kb region on chromosome 11 demonstrated that dCATCH-Seq was highly reproducible with ~95% capture specificity. Comprehensive analyses of sequencing data generated using the dCATCH-Seq approach also showed high accuracy in the detection of genetic variants and HLA typing. The double hybridization capture approach can also be coupled with bisulfite sequencing for DNA methylation profiling of both CpG and non-CpG sites. Altogether, dCATCH-Seq is a powerful and scalable targeted sequencing approach to investigate both genetic and epigenetic features.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 55%
Student > Ph. D. Student 5 23%
Lecturer > Senior Lecturer 1 5%
Other 1 5%
Student > Master 1 5%
Other 1 5%
Unknown 1 5%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 50%
Agricultural and Biological Sciences 8 36%
Nursing and Health Professions 1 5%
Chemistry 1 5%
Unknown 1 5%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2021.
All research outputs
#6,371,766
of 24,226,848 outputs
Outputs from BMC Genomics
#2,556
of 10,925 outputs
Outputs of similar age
#98,872
of 332,133 outputs
Outputs of similar age from BMC Genomics
#50
of 209 outputs
Altmetric has tracked 24,226,848 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 10,925 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 332,133 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 209 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.